Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling

doi: 10.12659/MSM.907277

Figure Lengend Snippet: Influence of ROCK1 on the expression and distribution of RhoA in LPS-treated HCECs. ( A ) Expression and distribution of ROCK1. ( B ) Expression and distribution of RhoA. Scale bar, 100 μm. Each assay was represented by three replicates.

Article Snippet: Antibodies against RhoA (cat. no. A00207), ROCK1 (cat. no. BM4203), and TLR4 (cat. no. BA1717) were purchased from Boster (China).

Techniques: Expressing

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling

doi: 10.12659/MSM.907277

Figure Lengend Snippet: Influence of ROCK1 on the activity of TLR4-mediated NF-κB and ERK signaling in LPS-treated HCECs. ( A ) ROCK1, RhoA, and members of TLR4-mediated NF-κB signaling. ( B ) Members of TLR4-mediated ERK signaling. Each western blotting assay was represented by three replicate and data were expressed as mean ± standard deviation.

Article Snippet: Antibodies against RhoA (cat. no. A00207), ROCK1 (cat. no. BM4203), and TLR4 (cat. no. BA1717) were purchased from Boster (China).

Techniques: Activity Assay, Western Blot, Standard Deviation

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling

doi: 10.12659/MSM.907277

Figure Lengend Snippet: Relative expression levels of proteins on western blotting assays.

Article Snippet: Antibodies against RhoA (cat. no. A00207), ROCK1 (cat. no. BM4203), and TLR4 (cat. no. BA1717) were purchased from Boster (China).

Techniques: Expressing, Western Blot, Control, Clinical Proteomics

Journal: PLoS ONE

Article Title: MiR-124 Promote Neurogenic Transdifferentiation of Adipose Derived Mesenchymal Stromal Cells Partly through RhoA/ROCK1, but Not ROCK2 Signaling Pathway

doi: 10.1371/journal.pone.0146646

Figure Lengend Snippet: (A) QRT-PCR analysis of miR-124 expression in ADMSCs infected with the pLV-miR-124 locker lentiviral particles or the vector negative control (Vector NC). (B) QRT-PCR analysis of NSE, Tuj-1 and GFAP mRNA expression in ADMSCs (with or without miR-124 knockdown) on day 1 post-induction. (C) Representative images of flow cytometry analysis of ADMSCs (with or without miR-124 knockdown) with positive neurogenic markers on day 1 post-induction. (D) Quantification of the proportion of ADMSCs with positive neurogenic markers showed in (C). * p <0.05, ** p <0.01, *** p <0.001.

Article Snippet: The ready-to-use human RhoA shRNA lentiviral particles (sc-44209-V), ROCK1 shRNA lentiviral particles (sc-29473-V) and ROCK2 shRNA lentiviral particles (sc-29474-V) were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Quantitative RT-PCR, Expressing, Infection, Plasmid Preparation, Negative Control, Knockdown, Flow Cytometry

Journal: PLoS ONE

Article Title: MiR-124 Promote Neurogenic Transdifferentiation of Adipose Derived Mesenchymal Stromal Cells Partly through RhoA/ROCK1, but Not ROCK2 Signaling Pathway

doi: 10.1371/journal.pone.0146646

Figure Lengend Snippet: (A) QRT-PCR (up) and western blot (down) analysis of RhoA expression in ADMSCs on day 0, 5 and 10 during the transdifferentiation induction. (B) Predicted binding site between 3’UTR of RhoA mRNA and miR-124 and the designed mutant sequence. (C) Dual luciferase assay of relative luciferase activity of pGL3-RhoA-WT (RhoA-WT) and pGL3-RhoA-MUT (RhoA-WT) transfected with 50 nM miR-124 mimics or the negative control in HEK 293T cells. Firefly luciferase activity was normalized to that of Renilla luciferase. (D) QRT-PCR (up) and western blot (down) analysis of RhoA expression in ADMSCs infected with the pLV-miR-124 locker lentiviral particles or the negative control. * p <0.05, ** p <0.01, *** p <0.001.

Article Snippet: The ready-to-use human RhoA shRNA lentiviral particles (sc-44209-V), ROCK1 shRNA lentiviral particles (sc-29473-V) and ROCK2 shRNA lentiviral particles (sc-29474-V) were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Binding Assay, Mutagenesis, Sequencing, Luciferase, Activity Assay, Transfection, Negative Control, Infection

Journal: PLoS ONE

Article Title: MiR-124 Promote Neurogenic Transdifferentiation of Adipose Derived Mesenchymal Stromal Cells Partly through RhoA/ROCK1, but Not ROCK2 Signaling Pathway

doi: 10.1371/journal.pone.0146646

Figure Lengend Snippet: (A) QRT-PCR analysis of RhoA, ROCK1 and ROCK2 mRNA expression in ADMSCs infected with the RhoA shRNA lentiviral particles, ROCK1 shRNA lentiviral particles, ROCK2 shRNA lentiviral particles or the negative control. (B) QRT-PCR analysis of NSE, Tuj-1 and GFAP mRNA expression in ADMSCs infected with the pLV-miR-124 locker lentiviral particles, co-infected with the pLV-miR-124 locker lentiviral particles and the RhoA shRNA lentiviral particles, ROCK1 shRNA lentiviral particles or ROCK2 shRNA lentiviral particles on day 1 after transdifferentiation induction. (C, E and G) Representative images of flow cytometry analysis of neurogenic markers (NSE, Tuj-1 and GFAP) positive ADMSCs infected with the pLV-miR-124 locker lentiviral particles and co-infected with the pLV-miR-124 locker lentiviral particles and the RhoA shRNA lentiviral particles (C), ROCK1 shRNA lentiviral particles (E) or ROCK2 shRNA lentiviral particles (G) on day 1 after transdifferentiation induction. (D, F and H) Quantification of the proportion of ADMSCs with positive neurogenic markers showed in C, E and G. *: comparison with shRNA NC or vector NC; #: comparison with transdiff. ADMSCs/miR-124(-). * and # p <0.05, ** and ## p <0.01, *** and ### p <0.001.

Article Snippet: The ready-to-use human RhoA shRNA lentiviral particles (sc-44209-V), ROCK1 shRNA lentiviral particles (sc-29473-V) and ROCK2 shRNA lentiviral particles (sc-29474-V) were purchased from Santa Cruz Biotech (Santa Cruz, CA, USA).

Techniques: Quantitative RT-PCR, Expressing, Infection, shRNA, Negative Control, Flow Cytometry, Comparison, Plasmid Preparation